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DeepClear™

Item No.:

DC-201

Price:

US$ 200

Spec.:



DeepClear is a tissue clearing solution with a reflection index of RI: 1.52 suitable for single cell imaging and

Introduction
 
DeepClear™ is non-toxic, ready to use and a water-soluble clearing agent for enhancing the transparency of cross-linking agent-fixed biological specimens and its clearing effect is able to reach up to a depth of 500 microns below the specimen surface. The Refractive Index (RI) of DeepClear™ is 1.52 which is higher than FocusClear™ and will greatly improve the distortion problem in the z-axis. Oil objective lenses with higher N.A. are capable of using DeepClear™. Immunostaining. With the use of DeepClear™ to more significantly increase the effectiveness of laser excitation and detection of color and fluorescence, obtained microscopic image quality will be greatly improved. Samples labeled with immunostaining, fluorescence and non-fluorescence dyes including lipophilic dyes, such as DiI, DiD and NBD-ceramide can be directly transferred from water, buffer solutions, alcohol, DMSO, DMF, and glycerin into FocusClear™ solution.
 
 
Highlights
  • A water-soluble clearing agent that increases the transparency of biological tissues.
     
  • Allows for visualization of internal objects up to 3 mm below the tissue surface. 
     
  • Increases depth of view by at least 12X over traditional glycerol-based mounting media. 
     
  • Increases the efficacy of laser excitation and optical signal detection of either color or fluorescence. 
     
  • Suitable for immunofluorescent-labeled single cells and tissues, in situ hybridization, tissue or whole-mount immunohistochemistry and fluorescent protein samples.

 

Product Specifications

 

Application
Animal: brain, kidney, heart, stomach, intestine, liver, lung, pancreas, skin
Insect: brain, ventral nerve cord, ovary
Biomaterial: collagen matrix
Plant
Clearing
Solitary use
Recommended
0.5~1mm depth tissue slice
With CLARITY*
Recommended Whole organ
With SCALEVIEW**
Not recommended
Clearing
Speed
Solitary use
Within 1 Hour
With CLARITY
Days
With SCALEVIEW
-
Immunostaining Compatibility
Yes
Fluorescent protein Compatibility
Yes
Organic dye Compatibility
Yes
Lipophilic dye Compatibility
No
Objective lens
Objective lens with high NA and long WD is suggested, 
 
Cat. No.
FC-101
FC-102
FC-103
Format
5ml
50ml
500ml
Storage
4~30
39~86

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