Introduction
 

DeepClear™ is non-toxic, ready to use and a water-soluble clearing agent for enhancing the transparency of cross-linking agent-fixed biological specimens and its clearing effect is able to reach up to a depth of 500 microns below the specimen surface. The Refractive Index (RI) of DeepClear™ is 1.52 which is higher than FocusClear™ and will greatly improve the distortion problem in the z-axis. Oil objective lenses with higher N.A. are capable of using DeepClear™. Immunostaining. With the use of DeepClear™ to more significantly increase the effectiveness of laser excitation and detection of color and fluorescence, obtained microscopic image quality will be greatly improved. Samples labeled with immunostaining, fluorescence and non-fluorescence dyes including lipophilic dyes, such as DiI, DiD and NBD-ceramide can be directly transferred from water, buffer solutions, alcohol, DMSO, DMF, and glycerin into FocusClear™ solution.