1. First STEP
Materials and devices for sample clearing and embedding: 5 ml each of FocusClear™, MountClear™ and Immersion Solution-M, a 24-well cleaning plate, cleaning paper, a cover slip, reinforcing rings, a loop and a clamp.
Before to begin sample clearing, put a few drops of FocusClear™ (about 100 μl) into as many wells of a 24-well plate as needed. If the thickness of specimen is over 400 microns, using an epen droff as the incubation chamber is recommended.