FocusClear™ is non-toxic, ready to use and a water-soluble clearing agent for enhancing the transparency of cross-linking agent-fixed biological specimens and its clearing effect is able to reach up to a depth of 500 microns below the specimen surface. With the use of FocusClear™ to significantly increase the effectiveness of laser excitation and detection of color and fluorescence, obtained microscopic image quality will be greatly improved. Samples labeled with fluorescence and non-fluorescence dyes including lipophilic dyes, such as DiI, DiD and NBD-ceramide can be directly transferred from water, buffer solutions, alcohol, DMSO, DMF, and glycerin into FocusClear™ solution.

 

 

Specification		Refractive Index: 1.45 pH: 11
Application		Animal: brain, kidney, heart, stomach, intestine, liver, lung, pancreas, skin Insect: brain, ventral nerve cord, ovary Biomaterial: collagen matrix Plant
Clearing	Solitary use	Recommended 0.5~1mm depth tissue slice
	With CLARITY*	Recommended Whole organ
	With SCALEVIEW**	Not recommended
Clearing Speed	Solitary use	Within 1 Hour
	With CLARITY	Days
	With SCALEVIEW	-
Immunostaining Compatibility		Yes
Fluorescent protein Compatibility		Yes
Organic dye Compatibility		Yes
Lipophilic dye Compatibility		Yes
Objective lens		Objective lens with high NA and long WD is suggested,  Leica HCX IRAPOL 25X water-immersion objective, NA 0.95, WD 2.4mm;  Zeiss 20X W-PlanApochromat objective, NA 1.0, WD 2.0 mm Olympus XLSLPLN25XSVMP objective, NA 0.9, WD 8.0 mm
Cat. No.		FC-101	FC-102	FC-103
Format		5ml	50ml	500ml
Storage		4℃~30℃ 39℉~86℉